Several assay approaches are employed to measure the ability of an agent to damage cells in vitro as an indicator for acute toxicity in vivo.
MTS Assay (mitochondrial activity)
The MTS cell proliferation assay is a colorimetric method to identify the cytotoxic potential of a test item. The assay measures the formation of a soluble formazan product which is directly proportional to the number of live cells in culture. This assay can be performed with HepG2 cells or L-929 cells.
LDH Leakage Assay (membrane integrity)
The LDH Leakage assay determines the membrane integrity/extent of LDH leakage from cells with a damaged membrane under the influence of test item. The assay is a fluorometric measure of the release of lactate dehydrogenase (LDH) from cells with a damaged membrane by conversion of resazurin into resorufin that is measured by change in absorbance. This assay can be performed with HepG2 or L-929 cells.
Multiple cell types may be used, depending on the pharmacological action, potential target, metabolism and mechanism, or end point of the agent.
Types of Cells Available:
- Human hepatoma HepG2 cells
- L-929 mouse fibroblasts
Tests conducted in compliance with GLP requirements according to the ISO 10993-5 guideline.